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. 2021 May 11;11:10012. doi: 10.1038/s41598-021-89476-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of the gene expression in a large cohort of 279 HNC cases from Cancer Genome Atlas containing HM450 methylation, RNAseq data as well as information regarding alcohol, tobacco, and HPV infection. CD44, CCNA1, DCC and TIMP3 were hypermethylated in the HNC HPV-negative cases. Image done using the open-access resource for interactive exploration of multidimensional cancer genomics data sets cBio Cancer Genomics Portal (http://cbioportal.org).