Fig. 8.

Characterization of NMDA receptor with antagonists. DoD24 MCCs were used for Ca²⁺ imaging and were preincubated with different antagonists of the NMDA receptor. a MCCs were pre-incubated for 30 min with different antagonists of the NMDA-R: MgCl₂ [5 mM], AP5 [50 µM], MK801 [6 µM], traxoprodil [5 µM], Pb(II)ac [50 µM], or Zn²⁺ [10 µM] followed by addition of 50 µM NMDA. The percentage of reacting cells from the whole cell population is shown. Data are means of three biological replicates ± SD, #p value < 0.05 (reaction vs NMDA alone). b Averaged traces from Ca²⁺ signalling of a. The full set of data with standard deviations is displayed in Fig. S6. c MCCs were pre-incubated for 30 min with phencyclidine [50 µM] (PCP) or dextromethorphan [50 µM] (DXM) followed by the addition of NMDA [50 µM]. The percentage of reacting cells compared to the whole cell population is shown. Data are means of three biological replicates ± SD. **p value < 0.01. d MCCs were pre-incubated for 30 min with phencyclidine [50 µM] (PCP) or dextromethorphan [50 µM] (DXM) followed by the addition of ibotenic acid [50 µM]. The percentage of reacting cells compared to the whole cell population is shown. Data are means of three biological replicates ± SD; **p value < 0.01