Table 2.
Primary antibody details
| Antigen | Species | Used for | Dilution | Antigen retrieval | Manufacturer | Cat. Number |
|---|---|---|---|---|---|---|
| CD19 | Rabbit | Human | 1:100 | EDTA-Tris pH 8 | Abcam | ab134114 |
| CD3 | Mouse | Human | 1:20 | EDTA-Tris pH 8 | DAKO | M7254 |
| CD3 | Mouse | Rat | 1:500 | without | BD Pharmigen | 550,295 |
| CD4 | Rabbit | Human | 1:150 | Citrate pH 6 | Abcam | ab133616 |
| CD68 | Mouse | Human | 1:100 | Citrate pH 6 | Abcam | Ab995 |
| CD79a | Mouse | Rat | 1:200 | without | ThermoFisher | MA5-13,212 |
| CD8 | Mouse | Human | 1:1000 | Citrate pH 6 | DAKO | M7103 |
| HLA class II (CR3/43) | Mouse | Human | 1:500 | Citrate pH 6 | DAKO | M077501-2 |
| HLA class II (OX6) | Mouse | Rat | 1:50 | without | In house | |
| HuC/D | Mouse | Human/Rat | 1:1000 | Citrate pH 6/EDTA-Tris pH 8 | ThermoFisher | A21271 |
| IBA1 | Goat | Human/Rat | 1:500 | Citrate pH 6/EDTA-Tris pH 8/without | Abcam | ab5076 |
| IBA1 | Rabbit | Human/Rat | 1:1000 | Citrate pH 6/EDTA-Tris pH 8/without | WAKO | 019–19,741 |
| LAMP1 | Rabbit | Human | 1:500 | Citrate pH 6 | Cell Signaling | 9091P |
| LAMP1 | Rabbit | Rat | 1:1000 | EDTA-Tris pH 8 | Abcam | ab24710 |
| MOG | Mouse | Human/Rat | 1:50 | Citrate pH 6 | Reynolds Lab | N.A |
| P2RY12 | Rabbit | Human | 1:100 | Citrate pH 6 | AnaSpec | 55042A |
| P2RY12 | Rabbit | Rat | 1:100 | without | AnaSpec | 55043A |
| Synaptophysin | Mouse | Human/Rat | 1:500 | Citrate pH 6/EDTA-Tris pH 8 | DAKO | M7315 |
| TMEM119 | Rabbit | Human | 1:500 | Citrate pH 6 | Atlas abcam | HPA051870 |
| vGAT | Mouse | Rat | 1:500 | EDTA-Tris pH 8 | Synaptic Systems | 131 011 |