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. 2021 May 11;4:555. doi: 10.1038/s42003-021-02058-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — T. b. brucei 1738 wild-type trypanosomes derived from tsetse salivary glands were fixed and stained with YL1/2 antibody against tyrosinated α-tubulin visualized with FITC secondary antibody; the basal bodies (BBs) shown as bright green fluorescent dots, with additional staining of the plus ends of newly formed microtubules at the posterior of the cell. L to R: phase contrast, YL1/2 (green FITC fluorescence), DAPI (false coloured magenta fluorescence), merge all. BBs are indicated by white arrows (YL1/2 images) and kinetoplasts (K) by arrowheads (DAPI images). a 1K1N epimastigote with prominent posterior nozzle; b 2K2N dividing epimastigote; c 2K1N meiotic divider, with just visible anterior BB; d 1K1N metacyclic; e 1K1N gamete; f 2K1N gamete. g–k various meiotic intermediates with multiple K and/or N: g 3K3N cell with two flagella; h 3K2N cell with two flagella; i 2K2N cell with two flagella; j 2K2N, single flagellum; k 3K2N, single flagellum. Scale bar = 5 µm.