Fig. 4. Morphology of meiotic intermediates from tsetse salivary glands.

Salivary gland-derived trypanosomes of 1738 H2B::GFP PFR1::YFP were fixed and stained with DAPI. L to R: phase contrast, GFP, merge, DAPI (magenta), merge all. a–j show individual trypanosomes with nuclei of different fluorescence intensities. a–e show trypanosomes with two nuclei of different fluorescence intensities; a, b show a single 2K2N cell where the smaller nucleus is posterior, while in c the smaller nucleus is anterior; d, e show dividing 2K2N cells with two flagella. f–j trypanosomes with three nuclei of different fluorescence intensities; f 3K3N cell, where the central nucleus appears larger than the other two; g–i show a 1K1N daughter cell with a small nucleus cleaving from a 2N trypanosome with two nuclei of different fluorescence intensities, while in j the 2K2N cell at the posterior is cleaving from a 1K1N gamete at the anterior, and the 2K2N “daughter” cell has nuclei of unequal fluorescence. k–p show the final stages of meiosis. k 3K1N cell with a single long flagellum; l–p gamete-like trypanosomes with two nuclei of equal intensity; two flagella are evident in cells m, n, p, but only a single flagellum is visible in cells l, o, so the daughter flagellum is either running closely parallel with the original or has not formed; o 1K1N gamete cleaving from the posterior of a 2K1N gamete, while in p cytokinesis is almost complete, yielding one 1K1N and one 2K1N gamete. Kinetoplasts are indicated by arrowheads. Scale bar = 5 µm.