Table 1.
Morphology of predominant types of meiotic intermediates among salivary gland-derived trypanosomes of T. b. brucei.
| Cell type (ordered by no. of nuclei) | J10 PFR1::YFP YFP::HOP1 and PFR1::YFP YFP::DMC1 |
1738 Mito GFP | 1738 H2B::GFP PFR1::YFP |
TREU 927 HAP2::YFP |
|---|---|---|---|---|
| N = 50 | N = 138 | N = 214 | N = 351 | |
| 3K1N | 0 (0%) | 20 (15%) | 5 (2%) | 67 (19%) |
| 2K2N | 8 (16%) | 32 (23%) | 57 (27%) | 54 (15%) |
| 3K2N | 11 (22%) | 21 (15%) | 50 (23%) | 73 (21%) |
| 4K2N | 12 (24%) | 14 (10%) | 33 (15%) | 46 (13%) |
| 3K3N | 6 (12%) | 10 (7%) | 18 (8%) | 11 (3%) |
Four data sets are listed, comprising data from salivary gland-derived trypanosomes of five different recombinant clones from three strains of T. b. brucei (J10, 1738, TREU 927). Overall, a total of 56 cytospins of pooled, dissected salivary glands were examined, derived from ~950 tsetse flies (J10, 12 cytospins, ~200 flies; 1738 Mito GFP, 5 cytospins, ~100 flies; 1738 H2B::GFP, 31 cytospins, ~500 flies; TREU 927, 8 cytospins, ~150 flies). Only the most numerous cell types (≥10%) in one or more trypanosome clones are shown; percentages relate to the total number of cells categorized (N).