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. 2021 Apr 30;20:1533033820980115. doi: 10.1177/1533033820980115

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — Alteration of OC cell malignancy after intervening YAP1 expression. (A) Effects of siRNA after being transfected were calculated by RT-qPCR, compared with control group, ***p < 0.001. Compared with si-YAP1-1,^## p < 0.01; (B) Western blot analysis was used in detecting YAP1 expression in differentially treated SKOV3, compared with control group, ***p < 0.001. Compared with miR-375 mimics/pcDNA3.1-NC, ^### p < 0.001; (C-E) scratch test, Transwell assay and flow cytometry were applied to determine biological behaviors in differentially treated cells respectively, compared with control group, *p < 0.05, **p < 0.01, ***p < 0.001. Compared with miR-375 mimics/pcDNA3.1-NC, ^### p < 0.001; one-way ANOVA and Tukey’s multiple comparisons test was applied to determine statistical significance, Repetitions = 3. YAP1: Yes-associated protein 1; OC: ovarian cancer; si: small interfere; RT-qPCR: reverse transcription quantitative polymerase chain reaction; miR: microRNA; ANOVA: analysis of variance.