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. 2021 Apr 28;15:652226. doi: 10.3389/fnins.2021.652226

FIGURE 2.

FIGURE 2

DNA methylation profiles of the SNCA intron 1 CGI in the bulk frontal cortex tissues. DNA from each bulk frontal cortex sample was bisulfite converted, and the methylation (%) of the individual CpGs was quantitatively determined by pyrosequencing. (A) Graph depicts the average percentage of DNA methylation level for 20 PD, 20 DLB, and 20 matched normal control subjects at each of the 23 CpG sites within the SNCA intron 1 CGI. (B) Box-and-scatterplot chart displays for each subject the DNA methylation average percentages across all 23 CpG sites within the SNCA intron 1 CGI [Chr4: 89,836,150-89,836,593 (GRCh38/hg38)] (denoted by colored points). The box plot also depicts the mean (denoted by colored horizontal line) and upper and lower quartiles (denoted by gray thin lines) of DNA methylation average percentages across all 23 CpG sites for all subjects in each group of PD, DLB, and normal control. The significance of the differences in % of methylation was determined using one-way ANOVA followed by Dunnett’s post hoc test and denoted by asterisks () for DLB vs normal, and by obelisks (†) for PD vs normal comparisons, with /†p 0.05, ∗∗/††p 0.01, and ∗∗∗/†††p 0.005.