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. 2021 May 11;12:2658. doi: 10.1038/s41467-021-22982-8

Fig. 4. In vivo functional characterization of glucosinolate transport mediated by PaGTR5/6/7/8.

Fig. 4

a PaGTR5/6/7/8 expression in P. armoraciae 6 days after dsRNA injection (n = 6 biological replicates, two beetles per replicate). b Accumulation of 2-propenyl glucosinolate (2Prop GLS) in adult P. armoraciae beetles after 6 days feeding on Arabidopsis leaves, followed by 1-day starvation. Box plots show the median, interquartile range, and outliers of each dataset (n = 10 biological replicates, six beetles per replicate). c Excreted amounts of 2Prop GLS from day 2 until day 6 of feeding on Arabidopsis (n = 10 biological replicates, feces of six beetles per replicate). Data are shown as mean ± s.e.m. Treatments were compared by two-tailed Student’s t test, or Mann–Whitney U test. n.s. Not significantly different; *P < 0.05, **P < 0.01, and ***P < 0.001. P values are provided in Supplementary Data 6.