Fig. 6. Ice recrystallization inhibition activity of peptides listed in Fig. 7.

a Mean grain area analysis of IRI activity of the peptides listed in Fig. 7. All IRI assays were performed in oxidizing conditions unless otherwise noted. Black squares represent the oxidized peptide 8. The red circle is reduced peptide 8. Orange triangles are Thr4-Ser mutation. Purple diamonds are Thr10-Ser mutation. Green triangles are Thr14-Ser mutation. Blue triangles all Thr have been mutated to Ser. Grey triangles is a scrambled peptide sequence. Teal hexagons Asp8-ser mutation. (values reported are averaged from three individual experiments and shown as mean data values ± SD. Error bars represent standard deviation). b Mean grain area IRI activity comparison of peptide 8 (black squares) to literature reported values for polyvinyl alcohol (PVA56) (red circles) and a truncated type I antifreeze protein 22 amino acids in length modified with anthracene (PC-AFP₂₂ monomer) (blue triangles)²⁹.