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. 2021 May 11;12:2705. doi: 10.1038/s41467-021-23055-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Overlap between genes with altered expression (RNA-seq) after 24 h of R1881 treatment and by Dtx3L knockdown in VCaP cells. The P value for the overlap is from a hypergeometric test (1.63-fold overrepresentation). For each treatment group, n = 3 biologically independent samples. b Genes increased and decreased (up, down arrows) in VCaP cells after R1881 treatment and Dtx3L knockdown intersected to identify genes that change significantly in both sets, comparing the Dtx3L effect −R1881 (left) and +R1881 (right). P values are from the Chi-squared test using a 2 × 2 contingency table for the distribution of overlapping genes. c Genes (%) activated or repressed by Dtx3L knockdown in VCaP cells that are activated or repressed by R1881. ***P < 0.001 determined by Chi-squared test of a 2 × 2 contingency table for comparison to the expected overlap for activated or repressed genes or all R1881-regulated genes. d For genes that are activated or repressed by R1881 the percentages that are affected by Dtx3L knockdown +R1881 in VCaP cells are shown. *P < 0.05, **P < 0.01, ***P < 0.001 for comparison to the expected overlap for activated or repressed genes or all Dtx3L-regulated genes. P values were determined by the Chi-squared test of a 2 × 2 contingency table. e The effect of Dtx3L knockdown on R1881-activated and repressed genes in VCaP cells is shown as the differential effect of R1881 between control and Dtx3L knockdown. f Genes with increased or decreased expression +R1881 (VCaP cells) were intersected with those genes with higher or lower expression (up, down arrows) in the top versus bottom decile (KT hi) of the TCGA PrAd data sorted by combined KLK3 and TMPRSS2 expression. P values as in panel b. g The percentage of Dtx3L-regulated genes differentially expressed (VCaP cells) in the top versus bottom decile (TCGA PrAd data) sorted by combined KLK3 and TMPRSS2 expression. P values (*P < 0.05, **P < 0.01, ***P < 0.001) were determined by Chi-squared test of a 2 × 2 contingency table. Source data are provided as a Source data file.