Fig. 5. Re-establishment of ACD by transient mTOR inhibition in naive CD8+ T cells from aged mice reflects in correlates with improved memory potential.
A Experimental setup (illustration by MB). Naive and TVM CD8+ T cells were sorted from young and old P14 mice spleens, stimulated under different conditions, harvested after 36 h and adoptively transferred into wild-type recipients. Each recipient mouse received 1 × 104 cells and was infected intravenously with LCMV-WE (200 ffu) 30 days later. B Numbers of adoptively transferred P14 cells within CD8+ T cells in the spleens of recipient mice. C Percentages and numbers of KLRG1hi IL-7Rαlo, KLRG1lo IL-7Rαhi and KLRG1hi IL-7Rαhi P14 cells in the spleens of recipient mice (left panel). Representative plots showing gating strategy used to access expression of KLRG1 and IL-7Rα (right panel). D Frequencies and numbers of IFNγ, TNF and IL-2 producing cells in the spleens of recipient mice. Pooled data from two independent experiments (young naive, n = 8; young naive R, n = 8; young TVM, n = 3; young TVM R, n = 3; old naive, n = 6; old naive R, n = 6; old TVM, n = 7; old TVM R, n = 8). Aged P14 animals used for adoptive cell transfer: 92 or 96 weeks (experiment 1); 70 or 75 weeks (experiment 2). Data are depicted as mean + SEM. Statistical analysis was performed using the unpaired two-tailed Student’s t test. Exact P values are depicted in the figure.