Fig. 3. Cell type proportion estimates for n = 5 simulated individuals (dark blue boxes) with a cell type of interest and n = 5 individuals without that cell type (light blue boxes).
Cell type proportions are simulated at (a) 0.1% (two-sided grouped t-test; 5×: n.s., 10×: n.s, 100×: n.s., 1000×: p = 3.5 × 10−5), (b) 0.5% (two-sided grouped t-test; 5×: n.s., 10×: p = 0.013, 100×: 2.1 × 10−6, 1000×: p = 5.7 × 10−11), (c) 1% (two-sided grouped t-test; 5×: n.s., 10×: p = 1.5 × 10−3, 100×: 2.8 × 10−9, 1000×: p = 4.3 × 10−12), or (d) 5% (two-sided grouped t-test; 5×: 4.8 × 10−8, 10×: p =5.4 × 10−9, 100×: 1.8 × 10−14, 1000×: p < 2.0 × 10−16). The true fixed percentage of the cases is indicated by a red dotted line. Significant differences between the groups are indicated by *(p < 0.05), **(p < 0.01), and ***(p < 0.001). The centerline of the box indicates the mean, the outer edges of the box indicate the upper and lower quartiles, and the whiskers indicate the maxima and minima of the distribution. Data is shown for 50 independent simulations.