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. 2021 May 11;12:2622. doi: 10.1038/s41467-021-22925-3

Fig. 10. Peripheral specific Y1R blockade improves metabolic outcomes via multiple signalling pathways.

Fig. 10

a, c Representative western blotting images of p-CREB and p-ERK protein levels in intrascapular BAT and inguinal WAT (WATi) of vehicle- or BIBO3304-treated wild-type mice for 8 weeks. p phosphorylated. 14-3-3 was used as a loading control. Images are representative of three independent experiments. b, d Phosphorylated-CREB (p-CREB) and p-ERK protein expression expressed as fold change to housekeeping gene 14-3-3 and relative to chow-fed control in vehicle- or BIBO3304-treated wild type mice for 7 weeks. Data are mean ± s.e.m, chow n = 3 (control: open grey; BIBO3304: grey); HFD control n = 3 (open blue), BIBO3304 n = 4 (p-CREB) or 5 (p-ERK) (blue). *p < 0.05, ***p < 0.001, two-way ANOVA with Sidak’s multiple comparisons test. e Schematic of optical window implanted over intrascapular BAT of Akt-FRET biosensor mice. Illustration was adapted from Servier Medical Art, licensed under the Creative Commons Attribution 3.0 Unported license. f Representative images of dynamic Akt activity changes after acute glucose challenge in BAT of Akt-FRET biosensor mice on a HFD treated with vehicle or BIBO3304 jelly for 4 weeks, and fluorescence lifetime is quantified in (g) unit: nanosecond (ns). Data are mean ± s.e.m, vehicle n = 4, BIBO3304 n = 3. *p < 0.05 compared to 0 time point, Kruskal–Wallis one-way ANOVA. Images are representative of three independent experiments. Source data are provided as a Source Data file.