Skip to main content
. 2021 May 11;12:2622. doi: 10.1038/s41467-021-22925-3

Fig. 5. Peripheral Y1R antagonism induces browning of white adipose tissue.

Fig. 5

a The mRNA levels of thermogenic and lipogenic markers in inguinal WAT (WATi) of chow- and HFD-fed wild-type mice on a daily administration with a jelly containing a vehicle or BIBO3304. b mRNA levels of beige adipocyte-related genes in WATi. c mRNA expression of PPARγ, adiponectin, and β3R in WATi of chow- and HFD-fed wild-type mice on a daily administration with a jelly containing vehicle or BIBO3304. Data (a, b, c) are mean ± s.e.m, n = 3 in chow control (open grey), and 5 in chow BIBO3304 (grey); n = 4 in HFD control (open blue), and 8 in HFD BIBO3304 (blue). *p < 0.05, **p < 0.01, two-way ANOVA with Sidak’s multiple comparisons test. d Western blot analyses of UCP1, PGC1α and p-ACC protein levels in the WATi of vehicle- or BIBO3304-treated mice. 14-3-3 was used as a loading control. Images are representative of three independent experiments. KD kilodalton. e Quantification of the protein levels normalized to total protein content in BAT or WATi. Data are mean ± s.e.m, n = 3 in chow (control: open grey; BIBO3304: grey) or 4 in HFD (control: open blue; BIBO3304: blue). ***p < 0.001, two-way ANOVA with Sidak’s multiple comparisons test. f Representative immunohistochemical images of UCP1 protein expression in WATi of wild-type mice on either vehicle or BIBO3304 jellies for 7 weeks. n = 3 per group. g The mRNA levels of thermogenic and adipogenic markers in inguinal WAT (WATi) of Y1R−/− mice compared to wild type mice. Data are mean ± s.e.m, n = 4 per group (WT: mustard; Y1R−/−: purple). #p < 0.05, ##p < 0.01, Y1R−/− versus wild type determined by two-tailed t test. h The mRNA levels of thermogenic genes in WATi of chow- and HFD-fed Y1R−/− mice given either vehicle jelly or BIBO3304 jelly daily for 7 weeks. Data are mean ± s.e.m, chow n = 4 (control: open purple; BIBO3304: open green), HFD n = 6 (control: purple; BIBO3304: green). #p < 0.05, ##p < 0.01, two-way ANOVA with Sidak’s multiple comparisons test. Source data are provided as a Source Data file.