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. 2021 May 11;12:2622. doi: 10.1038/s41467-021-22925-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Schematic illustration of the paradigm for phenotyping the tamoxifen-induced adipocyte-specific Y1R deletion mouse model. b Relative Y1R mRNA expression in inguinal WATi, epididymal WATe, BAT, skeletal muscle and the liver of Adipo^TMCre/+;Y1^lox/lox mice receiving either vehicle or tamoxifen determined by RT-PCR using RPL19 as a housekeeping gene. Data are mean ± s.e.m. n = 4 per group (vehicle: white; tamoxifen: mustard). *p < 0.05 vs vehicle in the same fat depot by two-tailed t test. c, g Body weight of vehicle- or tamoxifen-treated Adipo^+/+;Y1^lox/lox control mice on a standard chow diet or a HFD for 8 weeks. Data are mean ± s.e.m. Chow (c): vehicle n = 7 (open circle), tamoxifen n = 6 (filled circle); HFD (g): vehicle n = 6 (open square), tamoxifen n = 7 (filled square). *p < 0.05, two-way repeated measures ANOVA. d, h, Change of fat mass of vehicle- or tamoxifen-treated Adipo^+/+;Y1^lox/lox mice on a chow diet or HFD determined by DEXA at 12 weeks of age. Data are mean ± s.e.m. vehicle n = 6 (open bar), tamoxifen n = 7 (filled bar) in both chow (d) and HFD (h); *p < 0.05 by two-tailed t test. e, i Body weight of vehicle- or tamoxifen-treated Adipo^TMCre/+;Y1^lox/lox on a chow diet or HFD for 8 weeks. Data are mean ± s.e.m. Chow (e): vehicle n = 8 (open circle), tamoxifen n = 10 (filled circle); HFD (i): n = 7 per group (vehicle: open square; tamoxifen: filled square), p values by two-way repeated measures ANOVA. f, j Change of fat mass of vehicle- or tamoxifen-treated Adipo^TMCre/+;Y1^lox/lox mice on a chow or HFD determined by DEXA at 12 weeks of age. Data are mean ± s.e.m. Chow (f): vehicle n = 7 (open bar), tamoxifen n = 6 (filled bar); HFD (j): vehicle n = 6 (open bar), tamoxifen n = 10 (filled bar). *p < 0.05, two-tailed t test. k, l Glucose levels in an intraperitoneal glucose tolerance test (GTT) in Adipo^+/+;Y1^lox/lox mice (light brown, vehicle n = 7, open square; tamoxifen n = 8 filled square) or Adipo^TMCre/+;Y1^lox/lox mice (black, vehicle n = 7, open square; tamoxifen n = 12 filled square) on a HFD at 13 weeks of age. Data are mean ± s.e.m. *p < 0.05, two-way ANOVA. m, n Glucose levels in an insulin tolerance test (ITT) in Adipo^+/+;Y1^lox/lox (light brown, vehicle n = 7, tamoxifen n = 8) or Adipo^TMCre/+;Y1^lox/lox mice (black, vehicle n = 7, tamoxifen n = 11) on a HFD at 14 weeks of age. Data are mean ± s.e.m. p values by two-way ANOVA. ns non-significance. Source data are provided as a Source Data file.