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. 2021 May 11;12:2648. doi: 10.1038/s41467-021-22914-6

Fig. 3. TeLC-mediated inactivation of NTS-projecting PSTh neurons diminishes 2MT-evoked hypothermia and abrogates tail temperature increase.

Fig. 3

a Schematic of TeLC-mediated inactivation of PSTh neurons in wild-type mice. b Representative image showing TeLC-GFP-labeled NTS-projecting PSTh neurons (n = 5). c Time-lapsed thermal images of wild-type mice without (EGFP, up) or with (TeLC, bottom) inactivation of NTS-projecting PSTh neurons during 2MT treatment. Tail (d), skin (f), and core (h) temperature curves of EYFP-expressing (n = 5) and TeLC-expressing (n = 3) mice before and during 2MT treatment. Average tail (e), skin (g), and core (i) temperature changes of EGFP-expressing (n = 5) and TeLC-expressing (n = 3) mice during 2MT treatment. Two TeLC-expressing mice were not included in the analysis because of low virus transduction rate (Supplementary Fig. 3). d–i Data are mean ± SEM; two-side Student’s t test. Scale bar, 100 µm.