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. 2021 May 11;12:2672. doi: 10.1038/s41467-021-22618-x

Fig. 6. Knockdown of FUT8 in TNBC cells enhances T-cell proliferation and activation.

Fig. 6

a The indicated MDA-MB-231-B7H3KO cells were transiently transfected with FUT8 siRNA for 48 h. The knockdown efficiency was analyzed by immunoblotting. b The indicated MDA-MB-231-B7H3KO cells were transiently transfected with FUT8 siRNA for 48 h, then cocultured with CD3/CD28-activated human T-lymphocyte cells for another 6 h. Left: representative dot plots of the cleavage of caspase-3 in tumor cells measured by flow cytometry. Right: percentage of cleaved caspase-3+ tumor cells (n = 3 biological independent samples). c–g Left: representative dot plots of in vitro activation of T measured by FACS as CFSE dilution after 5 days, respectively in the presence of irradiated B7H3-WT or B7H3-8NQ re-expressing in MDA-MB-231-B7H3KO cells. Right: percentage of proliferating CD4+ T (c), activation of IL2+CD4+ T (d), IL2+CD8+ T (e), IFNγ+CD4+ T (f), and IFNγ+CD8+ T (g) (n = 3 biological independent samples). Error bars represent mean ± SD. The p value in b–g was determined by a two-tailed unpaired Student’s t test. NS, not significance. Data are representative of three independent experiments.