Fig. 2. pOXA-48_K8 fitness effects in a set of ecologically compatible wild-type enterobacteria.
a Relative values of growth curve parameters (plasmid-carrying/plasmid-free isogenic clones): area under the growth curve (AUC), maximum optical density (ODmax), and maximum growth rate (μmax), represented as boxplots for E. coli (red) and Klebsiella spp. (blue) isolates separately. Horizontal lines inside boxes indicate median values, the upper and lower hinges correspond to the 25th and 75th percentiles, and whiskers extend to observations within 1.5 times the interquartile range. Dots represent each relative value. Values <1 indicate a reduction in these parameters associated with plasmid acquisition. Four and six biological replicates of the growth curves were performed for the wild-type isolates and the transconjugants, respectively (see Supplementary Figs. 1 and 2). b Relative fitness (w) of plasmid-carrying clones compared with plasmid-free clones obtained by competition assays (red, E. coli; blue, Klebsiella spp. see “Methods” for details). Values <1 indicate a reduction in w due to plasmid acquisition; values >1 indicate an increase in w. Bars represent normalised relative fitness after subtracting the effect of pBGC (average result of five independent biological replicates of the competition pOXA-48-carrying vs. pBGC-carrying strains divided by the average result of five independent biological replicates of the competition pOXA-48-free vs. pBGC-carrying strains), and error bars represent the propagated standard error. Two horizontal lines indicate those clones showing significant costs or benefits associated with carrying pOXA-48 plasmid (Bonferroni corrected two-sampled t test, P < 0.05). The names of the clones for which the relative fitness was calculated using E. coli strain J53 carrying the pBGC vector, instead of the pBGC-carrying parental strain, are indicated in pink. Source data are provided as Source data files.