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. 2021 May 11;12:2674. doi: 10.1038/s41467-021-22987-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a ELISA of IL-1β, IL-18, and TNF-α in supernatants from mouse peritoneal macrophages silenced of YAP, treated with indicated stimuli (mean ± SD, two-way ANOVA with Bonferroni test, YAP siRNA vs. Ctrl siRNA, left panel: **P = 0.0023, ***P = 0.0007, ***P = 0.0005 in sequence; middle panel: *P = 0.0222, **P = 0.0033, **P = 0.0076 in sequence; n = 3 independent experiments). b Immunoblot analysis of supernatants (SN) or cell lysates (CL) from mouse peritoneal macrophages silenced of YAP, treated with indicated stimuli. c ELISA of IL-1β, IL-18, and TNF-α in supernatants of mouse peritoneal macrophages from Yap^fl/fl lyz2-Cre or Yap^fl/fl mice, then treated with indicated stimuli (mean ± SD, two-way ANOVA with Bonferroni test, Yap^fl/fl lyz2-Cre vs. Yap^fl/fl, left panel: ***P = 0.0003, **P = 0.0046, ***P = 0.0006 in sequence; middle panel: **P = 0.0021, **P = 0.002, **P = 0.0013 in sequence; n = 3 independent experiments). d Immunoblot analysis of supernatants (SN) or cell lysates (CL) of mouse peritoneal macrophages from Yap^fl/fl lyz2-Cre or Yap^fl/fl mice, then treated with indicated stimuli. e Immunoblot analysis of ASC oligomerization in cross-linked cytosolic pellets of mouse peritoneal macrophages from Yap^fl/fl lyz2-Cre or Yap^fl/fl mice, primed with LPS, and followed by stimulation with nigericin. f Representative images of ASC specks in LPS primed Yap^fl/fl lyz2-Cre or Yap^fl/fl peritoneal macrophages treated with indicated stimuli. ASC, green; nuclei, blue. White arrows indicate ASC specks. Scale bars, 10 μm (left). The percentage of cells containing an ASC speck was quantified (right). At least 100 peritoneal macrophages from each genotype were analyzed (mean ± SD, two-way ANOVA with Bonferroni test, Yap^fl/fl lyz2-Cre vs. Yap^fl/fl, right panel: **P = 0.0011, ***P < 0.0001 in sequence; n = 3 independent experiments). Similar results were obtained from three independent experiments. Source data are provided as a Source Data file.