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. 2021 May 11;12:2674. doi: 10.1038/s41467-021-22987-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Immunoblot analysis of lysates from HEK293T cells transfected with HA-tagged ubiquitin (HA-Ub), HA-tagged K27-linked ubiquitin (K27-Ub), Myc-NLRP3 and Flag- β-TrCP1, with or without His-YAP, followed by IP with anti-Myc, probed with anti-HA. b Immunoblot analysis of cell lysates from mouse peritoneal macrophages silenced of β-TrCP1 stimulated with LPS for 4 h or not, followed by IP with anti-NLRP3, probed with anti-Ub. c Immunoblot analysis of lysates from HEK293T cells transfected with Myc-NLRP3, Flag- β-TrCP1, with or without His-YAP. d Immunoblot analysis of lysates from HEK293T cells transfected with Myc-NLRP3, Flag- β-TrCP1, with or without His-YAP, followed by IP with anti-Myc, probed with anti-Flag. Quantification of relative Flag levels was shown in the bottom panel, which was normalized to IP-Myc. e Immunoblot analysis of lysates from Yap^fl/fl lyz2-Cre or Yap^fl/fl mouse peritoneal macrophages stimulated with LPS for 4 h or not, followed by IP with anti-NLRP3, probed with anti- β-TrCP1. Quantification of relative β-TrCP1 levels is shown in the bottom panel, which was normalized to IP-NLRP3. Similar results were obtained from three independent experiments. Source data are provided as a Source Data file.