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. 2021 May 11;12:2670. doi: 10.1038/s41467-021-22958-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Evaluation of SARS-CoV-2 spike expression in 293T Lenti-X cells transfected with pSpike and pSpike-C3 plasmid by flow cytometry. Data from one representative experiment of three independent experiments are shown. b Western blot (WB) of lysates from concentrated preparations of LV-Luc pseudotyped with wild-type spike (LV-Luc/Spike) or spike truncated (21aa) at the cytoplasmic tail (LV-Luc/Spike-C3). Low dose: 1 × 10⁵ reverse transcriptase (RT) units; High dose: 6 × 10⁵ RT Units. Control vector included LV-Luc pseudotyped with VSV.G envelope (LV-Luc/VSV.G). Data from one representative of three independent experiments are shown. c Infection of human and macaque epithelial cell lines and the control B cell line Raji incubated with decreasing concentrations of LV-Luc pseudotyping the full length (LV-Luc/Spike) or the truncated form (LV-Luc/Spike-C3) of the SARS-CoV-2 spike. Data are expressed as mean luciferase activity (RLU) of duplicates. Nd = not done. Source data are provided as a Source Data file.