Figure 3.

Repertoire analysis of SpA_KK-sorted memory B cells and unsorted peripheral B cells from healthy controls. (A) Isotype usage as a % of total in SpA_KK-sorted repertoire. (B) Light chain usage as a % of total in SpA_KK-sorted repertoire. (C) Gene usage as % of total clones identified in sequenced SpA_KK-sorted repertoire. Clones mapped to the VH3 gene family are highlighted between the two vertical dashed lines. (D) Gene usage as % of total clones identified in the total memory B cell repertoire of a healthy control donor (Control 1). (E) Proportion of VH3 encoded BCR in repertoire in the SpA_KK-sorted peripheral memory B cells compared to three individual healthy controls.