Figure 3.

Incorporation of miR-142BS, miR-223BS, and miR-652BS into rAAV1.OVA transgene vectors reduces macrophage and DC activation. rAAV1.OVA expressing vectors with or without miR-BSs (1 × 10¹¹ GCs/mouse) were delivered by i.m. injections into C57BL/6 mice. Four weeks after injection, cells were isolated from TAs, lymph nodes, spleens, and livers and stained for macrophage markers (CD11b+, F4/80+) and activated DCs (CD11c+, CD80+, CD86+) followed by flow cytometry analysis. Relative frequencies of macrophage populations (A–D) and activated DCs (E–H) are represented as box plots with means, first and third quartile boundaries, and whiskers indicating max and min values. (n = 5). Mock = AAV1.empty capsid. p values were determined by one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.