Figure 4.

miR-142BS-, miR-223BS-, and miR-652-mediated detargeting suppresses OVA specific CD8 T cell response. C57BL/6 male mice, six weeks old, were i.m. injected with mock, empty capsid, or rAAV1.OVA vectors with or without miR-BSs (1 × 10¹¹ GCs/mouse). Mice were sacrificed four weeks after treatment and cells were isolated from TAs, lymph nodes, spleens and livers. Cells were then stained for CD8 T cell markers or with anti-CD8a/H-2Kb SIINFEKL tetramer and quantified by flow cytometry (n = 5). Relative frequencies of CD8+ T cells (A–D) and OVA-specific CD8+ T cells (E–H) are depicted as box plots with means, first and third quartile boundaries, and whiskers indicating max and min values (n = 5). p values were estimated by one-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.