Figure 3.

Modulation of PV expression affects mitochondrial Ca²⁺ uptake in single isolated FDB fibers

(A and B) Resting [Ca²⁺]_mt (A) and peak [Ca²⁺]_mt (B) upon caffeine stimulation in WT and PV^−/− FDB muscle fibers. n ≥ 23.

(C) Representative traces of the experiment as in (A) and (B). The arrow indicates the addition of caffeine.

(D and E) Resting [Ca²⁺]_mt (D) and peak [Ca²⁺]_mt (E) upon caffeine stimulation in FDB muscle single fibers electroporated with either shLUC or shPV for 7 days. n ≥ 18.

(F) Representative traces of the experiment as in (D) and (E). The arrow indicates the addition of caffeine.

(G and H) Resting [Ca²⁺]_mt (G) and [Ca²⁺]_mt peak (H) upon caffeine stimulation in FDB muscle single fibers electroporated with either mCherry or PV-P2A-mCherry for 7 days. n ≥ 21.

(I) Representative traces of the experiment as in (G) and (H).

Data are presented as mean ± SEM. For data analysis, two-tailed, unpaired Student’s t tests was performed. ^∗p < 0.05; ^∗∗p < 0.01. The arrow indicates the addition of caffeine.

See also Figures S3 and S4.