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. 2021 May 4;35(5):109087. doi: 10.1016/j.celrep.2021.109087

Figure 4.

Figure 4

PV ablation alters mitochondrial density, volume, and distribution and increases the number of mitochondria-CRU pairs

(A and B) Representative longitudinal sections of EDL fibers from adult WT and PV−/− mice. Small black arrows indicate pairs of mitochondria at the I band, large black arrows point to Z lines, and the empty arrow indicates a longitudinally oriented mitochondrion.

(C and D) Representative images of the distribution of mitochondria in cross-sections of EDL fibers from WT and PV−/− mice. Scale bars represent 1 μm (A and C) and 0.5 μm (B and D). n = 2 mice (WT); n = 3 mice (PV−/−).

(E) Quantitative analyses of mitochondria population. Column A, mitochondria volume; columns B and C, number and size; column D, percentage of mitochondria misplaced at the A band; column E, number of mitochondria/CRU pairs. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Sample sizes are as follows: column A, 13 fibers from two WT mice and 25 fibers from three PV−/− mice, with two micrographs per fiber; columns B–D, 20 fibers from two WT mice and 30 fibers from three PV−/− mice, with five micrographs per fiber; and column E, 20 fibers from two WT mice and 30 fibers from three PV−/− mice, with five micrographs per fiber.