REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Actin (C-2)	Santa Cruz Biotechnology	Cat#sc-8432; RRID: AB 626630
Akt (pan) (C67E7)	Cell Signaling	Cat#4691S; RRID: AB_915783
Phospho-Akt (Ser473) (D9E)	Cell Signaling	Cat#4060S; RRID: AB_2315049
Calsequestrin	Thermo Fisher Scientific	Cat# PA1-913; RRID: AB_2071461
F(ab’)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555	Thermo Fisher Scientific	Cat#A-21430; RRID: AB_2535851
F(ab’)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488	Thermo Fisher Scientific	Cat# A-11070; RRID: AB_142134
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647	Thermo Fisher Scientific	Cat# A-21235; RRID: AB_2535804
GAPDH (G-9)	Santa Cruz Biotechnology	sc-365062; RRID: AB_10847862
GRP75 (D-9)	Santa Cruz Biotechnology	sc-133137; RRID: AB_2120468
IP3R-I/II/III (B-2)	Santa Cruz Biotechnology	sc-377518; RRID: AB_2637028
Laminin	Merk	Cat#L9393; RRID: AB_477163
Monoclonal ANTI-FLAG® M2 antibody produced in mouse	Merk	Cat# F3165; RRID: AB_259529
Myosin Heavy Chain Type IIA antibody (α-SC-71)	DSHB	Cat#SC-71; RRID: AB_2147165
Myosin Heavy Chain Type IIB antibody (α-BF-F3)	DSHB	Cat#BF-F3; RRID: AB_2266724
Myosin Heavy Chain Type I antibody (α-BAD5)	DSHB	Cat#BA-D5; RRID: AB_2235587
Parvalbumin	Abcam	Cat#ab11427; RRID: AB_298032
SERCA1 ATPase Monoclonal Antibody VE121G9	Thermo Fisher Scientific	Cat# MA3-912; RRID: AB_2061281
SERCA2 ATPase Polyclonal Antibody	Thermo Fisher Scientific	Cat#PA5-78837; RRID: AB_2745953
MCU	Merk	Cat#HPA016480; RRID: AB_2071893
MICU1	Merk	Cat#HPA037479; RRID: AB_2675495
Goat Anti-Mouse IgG (H L)-HRP Conjugate antibody	Bio-Rad	Cat# 170-6516, RRID:AB_11125547
Goat Anti-Rabbit IgG (H L)-HRP Conjugate antibody	Bio-Rad	Cat# 170-6515, RRID:AB_11125142
Bacterial and virus strains
E.Coli DH10 MultiBac	Geneva Biotech	N/A
Chemicals, peptides, and recombinant proteins
Caffeine	Merk	Cat#58-08-2
DMEM, high glucose, HEPES	Thermo Fisher Scientific	Cat#42430-082
DMEM, high glucose, pyruvate	Thermo Fisher Scientific	Cat#41966052
Penicillin/streptomycin	Euroclone	Cat#ECB3001
Fetal Bovine Serum, qualified, heat inactivated, E.U.-approved, South America Origin	Thermo Fisher Scientific	Cat#10500064
PBS, pH 7.2	Thermo Fisher Scientific	Cat#20012068
Trypsin 1x EDTA	Euroclone	Cat#ECB3053D
TMRM	Thermo Fisher Scientific	Cat#T668
Carbonyl cyanide 3-chlorophenylhydrazone (CCCP)	Merk	Cat#C2759
Bisbenzimide H 33258 Fluorochrome, Trihydrochloride	Merk	Cat#23491-45-4
Fura-2, AM, cell permeant	Thermo Fisher Scientific	Cat#F1221
BTS-Calbiochem, N-Benzyl- p-toluenesulphonamide	Merk	Cat#1576-37-0
Hyaluronidase from bovine testes	Merk	Cat#H4272
Laminin	Merk	Cat#11243217001
Collagenase A	Merk	Cat#10103586001
Tyrode’s Solution, Acidic	Merk	Cat#T1788
ProLong Glass Antifade Mountant	Thermo Fisher Scientific	Cat#P36982
TriZOL	Thermo Fisher Scientific	Cat#15596026
SuperScript® II Reverse Transcriptase	Thermo Fisher Scientific	Cat#18064014
Oligo(dT)12-18 Primer	Thermo Fisher Scientific	Cat#18418012
cOmplete Protease Inhibitor Cocktail	Merk	Cat#4693159001
PhosSTOP	Merk	Cat#4906845001
Mowiol® 4-88	Merk	Cat#9002-89-5
Critical commercial assays
Pierce BCA Protein Assay Kit	Thermo Fisher Scientific	Cat#23225
Applied Biosystems Power SYBR Green PCR Master Mix	Thermo Fisher Scientific	Cat#4368708
NuPAGE 10%, Bis-Tris, 1.0 mm, Mini Protein Gel, 10-well	Thermo Fisher Scientific	Cat#NP0301BOX
NuPAGE 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel, 10-well	Thermo Fisher Scientific	Cat#EA0375BOX
Nitrocellulose Membranes, 0.45 μm, 8 × 12 cm	Thermo Fisher Scientific	Cat# 77010
M.O.M.® (Mouse on Mouse) Immunodetection Kit	Vector Laboratories	Cat#BMK-2202
Experimental models: cell lines
HeLa	ATCC	Cat#CCL-2; RRID: CVCL_0030
Experimental models: organisms/strains
CD-1 IGS mouse	Charles River Laboratories	Cat#Crl:CD1(ICR)
C57BL/6J mouse	Charles River Laboratories	Cat#C57BL/6J (JAX Mice Strain)
C57BL/6J PV −/− mouse	N/A	Schwaller et al., 1999)
Oligonucleotides
Primers for RT-PCR, see Table S1	This paper	N/A
Recombinant DNA
pcDNA3.1-mtGCaMP6f	Mammucari et al., 2015	N/A
pcDNA3.1-D1ER	Palmer et al., 2004	N/A
pmCherry-N1	Clontech	Cat#632523
PV-P2A-mCherry	This paper	N/A
PV-flag-P2A-mCherry	This paper	N/A
pZac-U6-shLuc-ZsGreen	University of Pennsylvania Vector Core (Philadelphia, PA, United States)	N/A
pZac-U6-shLuc-mCherry	Mammucari et al., 2015	N/A
pZac-U6-shPV-mCherry	This paper	N/A
pZac-U6-shPV-ZsGreen	This paper	N/A
pZac-U6-shMCU-mCherry	Mammucari et al., 2015	N/A
Software and algorithms
Fiji	ImageJ	Schindelin et al., 2012
Prism®	GraphPad	RRID:SCR_002798