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. 2021 Apr 28;15:643287. doi: 10.3389/fnana.2021.643287

FIGURE 1.

FIGURE 1

TRPM4 expression in CA1 pyramidal neurons at P35. Confocal images showing double labeling of (A) TRPM4 (Alexa 488, green, i) with MAP2 (Alexa 546, magenta, ii), merged signals (iii), in the whole hippocampal area. (B) Shows the expression of TRPM4 (i) MAP2 (ii) and the merged signals (iii) in area CA1, and (iv) shows a zoomed area (white rectangle); yellow arrowheads show neurons in the stratum pyramidale and the distribution in the soma and proximal apical dendrite, white arrowhead show the distribution in the stratum oriens, showing the expression in the somatic region of the neurons. (C) Shows the expression of TRPM4 (Alexa 488, green, i) and AnkG (Alexa 546, magenta, ii) and the merged signals (iii), and (iv) shows a zoomed area (white rectangle). (D) Graph of normalized fluorescence intensity of TRPM4 in S.P, S.R, and S.O of area CA1. Fluorescence intensity values were normalized to CA1 S.P for each mouse. Each point corresponds to an individual mouse (Kruskal–Wallis followed by Dunn’s post hoc test vs. CA1 S.P; n = 5 mice). S.P, stratum pyramidale; S.R, stratum radiatum; and S.O, stratum oriens. Calibration bars = 100 μm in (A), and 20 μm in (B,C).