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. 2021 May 3;296:100654. doi: 10.1016/j.jbc.2021.100654

Figure 2.

Figure 2

Effect of cobalamin on the ATPase activity of ABCD4.A, ATPase activity of reconstituted ABCD4 was measured in the presence of 1 mM cobalamin inside or outside of the liposomes. Proteoliposomes with or without cobalamin containing ABCD4 (6.76 μg or 5.08 μg, respectively) were incubated with ATP at 37 °C for 30 min. Error bars indicate the standard deviation (n = 3). Differences among the conditions were considered significant when p < 0.05 based on Student's t test (∗p < 0.05). B, ATPase activity of reconstituted ABCD4 was measured in the presence of various concentrations of cobalamin in the liposome lumen. Error bars indicate the standard deviation (n = 3), when not shown, fall within the symbol.