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. 2021 May 3;296:100654. doi: 10.1016/j.jbc.2021.100654

Figure 3.

Figure 3

Cobalamin transport activity of ABCD4.A, ABCD4-liposomes containing 100 μM of cobalamin were prepared. Proteoliposomes containing ABCD4 wildtype or K427A (5.54 μg or 4.95 μg, respectively) were incubated with ATP at 37 °C. The amount of cobalamin transported from inside of the liposomes at each time point was calculated by subtraction of the amount of cobalamin in the liposomes in each incubation from that without any incubation. The cobalamin transport activity of wildtype ABCD4 (•), ABCD4 (K427A) (▲), and the negative control (○) are shown. Error bars indicate the standard deviation (n = 3), when not shown, fall within the symbol. B, cobalamin containing ABCD4-liposomes with different internal pH conditions was incubated with ATP, and then the amount of cobalamin inside of the liposomes was quantified as in (A). Proteoliposomes at pH 5.5 or pH 7.5 containing ABCD4 (10.1 μg or 10.2 μg, respectively) were used for this assay. Error bars indicate the standard deviation (n = 3), when not shown, fall within the symbol.