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XIST attenuates high glucose-induced apoptosis in the Schwann cells. (A–D) The RSC96 cells are treated with HG, or co-treated with HG and pcDNA-3.1-XIST overexpression vectors. (A) The expression of XIST is measured by qPCR assays. (B,C) The apoptosis is detected by flow cytometry analysis. (D,E) The expression of Bax, caspase3, cleaved-caspase3, caspase9, and cleaved-caspase9 is assessed by Western blot analysis. N = 3, mean ± SD, *P < 0.05, **P < 0.01.
