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. 2021 Apr 28;8:655157. doi: 10.3389/fmolb.2021.655157

FIGURE 6.

FIGURE 6

MiR-30d-5p is able to target SIRT1 in the Schwann cells. (A) Bioinformatic analysis using Targetscan (http://www.targetscan.org/vert_72/) shows the interaction of SIRT1 with miR-30d-5p. (B–D) The RSC96 cells are treated with control mimic or miR-30d-5p mimic. (B) The luciferase activities of wild-type SIRT1 (WT) or mutant SIRT1 (MUT) are detected by luciferase reporter gene assays. (C) The expression of SIRT1 is examined by qPCR assays. (D) The expression of SIRT1 is examined by Western blot analysis. (E) The RSC96 cells are treated with control shRNA or XIST shRNA, or co-treated with XIST shRNA and miR-30d-5p inhibitor. The expression of SIRT1 is examined by Western blot analysis. N = 3, mean ± SD, *P < 0.05, **P < 0.01.