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. 2021 Apr 28;12:609693. doi: 10.3389/fphar.2021.609693

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Copyright © 2021 Zhao, Li, Gao, Xu, Zhu, Zhai, Zhang, Shen, Di, Wang, Chen, Huang, Sun and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BBR attenuates neuronal apoptosis in ischemic penumbra induced by CIRI. (A), TUNEL staining was performed on slices from cortical ischemic penumbra. (B), Ratio of positive cells. Original magnification is 400×, scale bars is 20 μm. (C), The apoptotic protein cleaved Caspase-3 from cortical ischemic penumbra was determined by Western blot. GAPDH was used as the loading control. n = 6. (D) Bar graph showing the statistical comparison of Caspase-3 protein levels across groups. Data are shown as the mean ± SEM. *p < 0.05 compared with Sham group; ^# p < 0.05 compared with the CIRI group.