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. 2021 Apr 27;12:644281. doi: 10.3389/fphar.2021.644281

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Copyright © 2021 Sultan, Alghetaa, Mohammed, Abdulla, Wisniewski, Singh, Nagarkatti and Nagarkatti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AEA decreases T cell subpopulations in the lungs and spleens. Mice were treated with SEB and AEA as described in Figure 1 legend. Each panel shows a representative experiment depicting lung MNCs and splenocytes analyzed for various T cell markers. Data from five mice/group is presented in the form of vertical bars with Mean+/-SEM. Panels A–D and E-H show data from the lungs and spleens, respectively. (A,E) CD3⁺CD4⁺ T cells (B,F) CD3⁺CD8⁺ T cells, (C,G) CD3+Vβ8+ T cells, (D,H) CD3+NK1.1 + cells. Five mice in each group were used and the data was confirmed in three independent experiments. *p ≤ 0. 05, **p ≤ 0. 01, ***p ≤ 0. 001.