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. 2021 Apr 27;12:644281. doi: 10.3389/fphar.2021.644281

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Copyright © 2021 Sultan, Alghetaa, Mohammed, Abdulla, Wisniewski, Singh, Nagarkatti and Nagarkatti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AEA suppresses T cell activation markers in splenocytes activated with SEB. The spleen cells were pretreated with AEA and then activated with SEB in vitro for 48 h as described in Figure 3 legend. The cells were stained for various activation markers. Each panel shows a representative experiment using flow cytometry, and the vertical bars depict percentage data from groups of five mice with Mean+/-SEM. (A) CD3⁺CD69 ⁺ cells, (B) CD3⁺ cells, (C) CD69 + 25+ cells, (D) CD69 ⁺ cells, (E) CD44 ⁺ cells, (F) CD62L + cells. *p ≤ 0. 05, **p ≤ 0. 01, ***p ≤ 0. 001.