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. 2021 Apr 27;12:644281. doi: 10.3389/fphar.2021.644281

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Copyright © 2021 Sultan, Alghetaa, Mohammed, Abdulla, Wisniewski, Singh, Nagarkatti and Nagarkatti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of select miRNAs and targeted genes. Mice were treated with SEB and AEA as described in Figure 1 legend. Mononuclear cells from the lungs of both groups were isolated and screened for expression of miRNA expression with their targeted genes by qRT-PCR. (A) Binding affinity between miRNA 23a-3p and targeted genes including ARG1 and TGFβ2. (B) miRNA 23a-3p expression. (C,D) Expression of ARG1 and TGFβ2. (E) Binding affinity between miRNA 34a-5p and targeted gene FOXP3. (F) Expression of miRNA 34a-5p. (G) Expression FOXP3. Statistical significances as p ≤ 0. 05, **p ≤ 0. 01, ***p ≤ 0. 001.