FIGURE 8.

AEA induces MDSCs and Tregs in the lungs of SEB administered mice. Mice were treated with SEB and AEA as described in Figure 1 legend. The lung MNCs were next stained for markers to detect MDSCs and Tregs. Each panel shows a representative experiment depicting lung MNCs analyzed for various T cell markers. Data from five mice/group is presented in the form of vertical bars with Mean+/-SEM. (A) Cells double-stained for CD11b and Gr1. a representative experiment using flow cytometry. (B) Cells double-stained for LY6C and LY6G. (C) Cells double-stained for CD4 and FOXP3. (D) Cells double-stained for CD4 and IL10. (E) Cells from the spleens were double-stained for CD11b and Gr1. Vertical bars show data from five mice. (F) AEA-induced MDSCs were incubated with splenic T cells that were activated with ConA at different ratios to create different Tcell:MDSC ratios. T cell proliferation was assessed by ³H- Thymidine Incorporation Assay. Data from five mice/group is presented in the form of vertical bars with Mean+/-SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.