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. 2021 Apr 28;12:627568. doi: 10.3389/fimmu.2021.627568

Figure 1.

Figure 1

Recognition of SARS-CoV-2/OC43 homologous peptides in γ-IFN ELISpot assays. PBMCs were stimulated with SCoV-DP15 and IL-2 and after 9 to 14 days outgrowing cells were tested for recognition of SCoV-DP15 or shorter peptides within SCoV-DP15 at a final peptide concentration of 20 µg/ml. Peptide-stimulated T-cell lines without addition of peptide in the ELISpot served as negative controls to assess unspecific background reactions. Shown are SFUs per 50µl of T-cell suspensions in γ-IFN ELISpot assays. (A) Recognition of SCoV-DP15 or of truncated peptides by DP-15-stimulated T-cell lines from four healthy SARS-CoV-2 seronegative subjects (B) Magnitude of SCoV-DP15-specific response of DP-15-stimulated T-cell lines from healthy (n=3) and HIV-1-infected (n=35) CoV-DP15-responders. Shown are SFUs after subtraction of background with indication of median, range and 1st and 3rd quartile. (C) SCoV-DP15-specific γ-IFN responses of SARS-CoV-2 convalescent patient #49 and #50. 50µl of T-cells were used of donor #49, 1x105 cells/well were used for donor #50. w/o. P, without peptide; SFUs, Spot Forming Units, SCoV-DP15, DLSPRWYFYYLGTGP; SCoV-DL11, DLSPRWYFYYL; OC43-QL11, QLLPRWYFYYL; CoV-WP10, WYFYYLGTGP; SCoV-DY9, DLSPRWYFY.