Figure 7.

T-cell recognition of peptides CoV-PT10 and CoV-WP10. (A) 50µl each of the SCoV-DP15-recognizing T-cell line from Donor 7 were stimulated in a γ-IFN ELISpot assay with peptides SCoV-DP15, CoV-PT10 or CoV-WP10 at a final concentration of 20µg/ml. Shown are SFUs per well after subtraction of background values in wells without addition of peptide. (B) 1x10⁵ cells of the T-cell line from donor 6 were incubated in a γ-IFN ELISpot assay with peptides D15, CoV-PP12, SCoV-DG12, CoV-RP11, SCoV-DL11, CoV-WP10, CoV-PT10, CoV-WT8 at a final concentration of 20µg/ml. Responses are given as SFUs per 1x10⁵ cells after subtraction of SFUs of wells without peptide.