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. 2021 Apr 28;11:640823. doi: 10.3389/fcimb.2021.640823

Figure 1.

Figure 1

Verification of AOL_s00004g335 knock-out mutants. (A) PCR verification of knock-out mutants. +, positive control. -, negative control. M, DNA marker. No. 14, 15, and 24 are positive transformants. (B–D) Verification of the knock-out mutants by measuring the hyphal growth on the TG medium containing different amounts of MG. 1, 2, 4 μl of MG solutions per 5 ml medium were used in (B–D), respectively. WT, wild-type strain. Δg335-14, Δg335-15, and Δg335-24: AOL_s00004g335 knock-out mutants.