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. 2021 Apr 28;11:640823. doi: 10.3389/fcimb.2021.640823

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Copyright © 2021 Long, He, Tan, Yang, Zhou, Liu, Mo and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of ammonia resistance of mutant strains on WA medium. (A) Determination of conidial germination rates of mutant and wild-type strains at 4, 8, and 12 hours. (B) Determination of conidial germination rates (24 h) of mutant and wild-type strains on WA medium containing different volume of MG. (C) Determination of the fungistatic effect of ammonia on the conidial germination rates (24h) of mutant and wild-type strain. (D) Exogenous MG relieved the fungistatic inhibition of ammonia on the conidial germination of wild-type strain. WT, wild-type strain. Δg335-14, Δg335-15, and Δg335-24: AOL_s00004g335 knock-out mutants. **P < 0.01.