| Gene edit | Guide RNA and location (Chr and strand) | Repair template | Primers |
|---|---|---|---|
| Trem2 R47H | gRNA-3 ACTGGGGGAGACGCAAGGCC Chr17:48548344 (+1) |
CTGCAGGGCATGGCCGGCCAGTCCTTGAGGGTGTCATGTACTTATGACGCCTTGAAGCACTGGGGGAGACaCAAGGCCTGGTGTCGGCAGCTGGGTGAGGAGGGCCCATGCCAGCGTGTGGTGAGCACACACGGTGTGTGGCTGC | Trem2 PCR_F: GTGGAAGGTACCCAAGGACC Trem2 PCR_R: CAGGCACCTACAAGTAGCCC Trem2 Seq_F: TCTCCTCTGCAGCCCTGTC Trem 2 Seq_R: AGCTACTGTGTACTCACCCTC. |
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