Table 1.

Kinetics of spontaneous and RutC-catalyzed deamination of 3-AA

Parameter	None	RutC	RutCR104A
Vmax	9.0 ± 1.0	31.9 ± 3.9	9.0 ± 0.9
Km (mM)	7.1 ± 1.6	9.1 ± 1.9	7.3 ± 1.4
kcat	N/A	52	15

N/A, not applicable.

Assays included RutB (5 μM), YdfG (1 μM), and the indicated RutC protein (3 μM when present) in Tris–HCl (40 mM, pH 8.2). Further details of the assay are included in the Experimental procedures section. V_max is reported as nmoles of NADPH consumed min⁻¹, k_cat is reported as min⁻¹ and was not applicable for the spontaneous reaction. Standard error is reported.