Table 2.
Strains, plasmids, and primers
| Strain | Genotype | Source |
|---|---|---|
| NCM3722 | E. coli K12 | (9) |
| DM13778 | K12/pSU18 | This study |
| NCM4105 | K12 ΔrutC | (9) |
| DM17047 | K12 ΔrutC/Pdm1666 | This study |
| DM13779 | K12 ΔrutC/Pdm1667 | This study |
| DM13743 | K12 ΔrutC/Pdm1668 | This study |
| NCM4861 | K12 ΔrutD | (9) |
| DM13746 | ΔrutD/pSU18 | This study |
| DM13747 | ΔrutD/pDM1667 | This study |
| DM17308 | ΔrutD/pDM1661 | This study |
| DM15418 | S. enterica LT2/pCV1 | Laboratory collection |
| DM15419 | S. enterica LT2 ridA3::MudJ/pCV1 | Laboratory collection |
| DM15421 | S. enterica LT2 ridA3::MudJ/DM1439 | Laboratory collection |
| DM17321 | S. enterica LT2 ridA3::MudJ/pDM1663 | This study |
| DM17322 | S. enterica LT2 ridA3::MudJ/pDM1664 | This study |
| BL21-AI | Laboratory collection | |
| DH5α | Laboratory collection | |
| DM16701 | K12 strain AG1/pCA24N-ydfG | (23) |
| Plasmid | Description | Source |
| pSU18 | Expression vector, CmR | (24) |
| pDM1666 | pSU18-rutC | Laboratory collection |
| pDM1667 | pSU18-rutCDEFG | Laboratory collection |
| pDM1668 | pSU18-rutC(310–312,CGA→GCA)DEFGG | Laboratory collection |
| pDM1661 | pSU18-rutC(310–312,CGA→GCA)D(Δ46–750)EFGG | This study |
| pCV1 | Arabinose-inducible expression vector, AmpR | (21) |
| pDM1439 | pCV1-ridA (Salmonella enterica) | Laboratory collection |
| pDM1663 | pCV1-rutC | This study |
| pDM1664 | pCV1-rutC(310–312;CGA→GCA); encodes RutCR104A | This study |
| BspQI-Modified pET28b | Expression vector for C-terminal His6, KanR | (22) |
| pDM1405 | Modified pET28b-rutC | Laboratory collection |
| pDM1638 | Modified Pet28b-rutC(310–312;CGA→GCA); encodes RutCR104A | This study |
| pCA24N-ydfG | Expression vector encoding E. coli ydfG | (23) |
| Primer | Sequence | |
| PR42 | 5′-NNGCTCTTCNATGCCAAAATCCGTAATTATTCCC-3′ | |
| PR43 | 5′-NNGCTCTTCNGTGCTTGGCGATATGCG-3′ | |
| PR1513 | 5′-NNGCTCTTCNTTCATGCCAAAATCCGTAATTATTCCC-3′ | |
| PR1513 | 5′-NNGCTCTTCNTTATCACTTGGCGATATGCGC-3′ | |
| PR1511 | 5′-TTACTCAACGGGCTTGCCAG-3′ | |
| PR1512 | 5′-TACGGGCGCATCAGCATAAGG-3′ | |
Strains are Escherichia coli unless noted otherwise.