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. 2021 May 3;2(2):100520. doi: 10.1016/j.xpro.2021.100520

Table 2.

Digestion reaction to linearize the sgRNA-containing plasmid (pU6-DHFR + GRA sgRNAs library)

Reagent Amount per reaction Final concentration
sgRNA-containing plasmid 100 μg 1 μg/μL
AseI 50 units 0.5 unit/μL
10× NE Buffer (3.1) 10 μL 1×
UltraPure water (DNase free) up to 100 μL n/a