Table 3.
Recipe for CytoMix (500 mL total), storage at 4°C
| Reagent | Final Concentration | Volume | 
|---|---|---|
| KPO4 (0.2 M) pH 7.6 | 10 mM | 25 mL | 
| KCl (2.5 M) | 120 mM | 24 mL | 
| MgCl2 (0.1 M) | 5 mM | 25 mL | 
| CaCl2 (1 M) | 0.15 mM | 75 μL | 
| HEPES (1 M) | 25 mM | 12.5 mL | 
| EGTA (0.1 M) pH 7.6 | 2 mM | 10 mL | 
| KOH (1 N) | titration to get pH 7.6 | N/A | 
| ddH2O | N/A | N/A | 
| Total | N/A | 500 mL | 
| ATP (100 mM) | 2 mM | N/A | 
| GSH (100 mM) | 5 mM | N/A |