Table 5.
Parasite Selection Medium (M2 medium), storage at 4°C
| Reagent (stock concentration) | Storage temperature | Final Concentration | Volume |
|---|---|---|---|
| DMEM | 4°C | N/A | 423.5 mL |
| FBS | −20°C | 10% | 50 mL |
| L-Glutamine (200 mM) | −20°C | 2 mM | 5 mL |
| Penicillin/Streptomycin (10,000 μg/mL each) | −20°C | 100 μg/mL | 5 mL |
| HEPES (1 M) | −20°C | 10 mM | 5 mL |
| Non-Essential Amino Acids (100×) | 4°C | N/A | 5 mL |
| Sodium Pyruvate (100 mM) | 4°C | 1 mM | 5 mL |
| Gentamicin (10 mg/mL) | 4°C | 10 μg/mL | 1 mL |
| aChloramphenicol (40 mg/mL) | −20°C | 40 μg/mL | 0.5 mL |
| aPyrimethamine (100 mM) | 4°C | 1 μM | 5 μL |
| Total | N/A | N/A | 500 mL |
a
M3 medium= M2 medium without chloramphenicol and pyrimethamine, storage at 4°C.