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Number of PCR reactions per sample
| Origin of genomic DNA | Number of PCR reactions | Genomic DNA amount per reaction |
|---|---|---|
| sgRNA-containing plasmid | 2 | 1 μg |
| Parasites from the first lysis in HFFs | 6 | 1 μg |
| Parasites from the inoculum | 6 | 1 μg |
| Organ/peritoneal lavage | Use all genomic DNA available | 1 μg |
| Parasites from organ/peritoneal lavage cultures on HFFs | 3 | 1 μg |
