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. 2021 May 3;2(2):100520. doi: 10.1016/j.xpro.2021.100520

Table 9.

PCR reaction mix

Reagent Final concentration Final volume
5 × Q5 buffer 1 × 20 μL
dNTP (10 mM) 200 μM 2 μL
P1 (10 μM) 0.5 μM 5 μL
aP2 (10 μM) 0.5 μM 5 μL
Template DNA 1 μg of genomic DNA or 500 ng of sgRNA-containing plasmid N/A
Q5 High-Fidelity DNA Polymerase N/A 1 μL
UltraPure (DNase free) water N/A up to 100 μL
a

Each sample has a specific P2.